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李广悦

      

李广悦,男,1983年10月出生,河北沧州人,分子酶学专家。现任1zplay比分网研究员、博士生导师。2007年获得河北农业大学生物技术专业学士学位,2010年获得1zplay比分网硕士学位,2014年获得中国科学院天津工业生物技术研究所博士学位。2014-2017年在德国马普煤炭所 Manfred.T.Reetz教授实验室从事博士后研究。2017年底回国,入职1zplay比分网。

李广悦主要从事新型生物防治酶的筛选和进化,并应用于农作物的病害防治中,服务于农业生产。已在ACS Catalysis、Chemical Science、Chemical Communications等SCI杂志上发表文章18篇,累计影响因子110。


已发表文章
1、Guangyue Li?, Hui Zhang?, Zhoutong Sun, Xinqi Liu and Manfred T. Reetz*. Multiparameter Optimization in Directed Evolution: Engineering Thermostability, Enantioselectivity, and Activity of an Epoxide Hydrolase. ACS Catalysis [J]. 2016, 6(6), 3679?3687. (IF5year =10.945)
2、Guangyue Li?, Peiyuan Yao?, Rui Gong?, Jinlong Li, Pi Liu,Richard Lonsdale, Qiaqing Wu, Jianping Lin,Dunming Zhu and Manfred T. Reetz*. Simultaneous engineering of an enzyme’s entrance tunnel and active site: The case of monoamine oxidase MAO-N. Chemical Science [J]. 2017, 8,4093-4099. (IF5year=8.733)
3、Guangyue Li?, Jie Ren?, Peiyuan Yao,Yitao Duan, Hailing Zhang, Qiaqing Wu, Jinhui Feng, Peter CK Lau*, Dunming Zhu*. Deracemization of 2-Methyl-1, 2, 3, 4-tetrahydroquinoline Using Mutant Cyclohexylamine Oxidase Obtained by Iterative Saturation Mutagenesis. ACS Catalysis [J]. 2014, 4,903-908. (IF5year =9.174)
4、Guangyue Li, Miguel A. Maria-Solano, Adrian Romero-Rivera, Sílvia Osuna and Manfred T. Reetz*. Inducing High Activity of a Thermophilic Enzyme at Ambient Temperatures by Directed Evolution. Chemical Communications [J]. 2017, 53,9454-9457. (IF5year =6.238)
5、Guangyue Li and Manfred T. Reetz*. Learning Lessons from Directed Evolution of Stereoselective Enzymes. Org. Chem. Front [J]. 2016. 3, 1350-1358 (IF5year =4.96)
6、Guangyue Li?, Peiyuan Yao?, Peiqian Cong, Jie Ren, Lei Wang, Jinhui Feng, Peter C.K. Lau, Qiaqing Wu*,and Dunming Zhu*. New recombinant cyclohexylamine oxidase variants for deracemization of secondary amines by orthogonally assaying designed mutants with structurally diverse substrates. Scientific reports [J]. 2016,6:24973. (IF5year =4.847)
7、Guangyue Li, M.J. L. J. Fürst, H. R. Mansouri, A. K. Ressmann, A. Ilie, F. Rudroff, M. D. Mihovilovic, M.W. Fraaije and M. T. Reetz, Org Biomol Chem, 2017,15,9824-9829. (IF5year =3.56)
8、Guangyue Li, Jianbo Wang and Manfred T. Reetz*. Biocatalysts for the pharmaceutical industry generated by structure-guided directed evolution of stereoselective enzymes. Bioorg. Med. Chem [J]. 2017, DOI: org/10.1016/j.bmc.2017.05.021 (IF5year=2.88)
9、Guangyue Li, Jie Ren, Hiroaki Iwaki, Dalong Zhang, Yoshie Hasegawa, Qiaqing Wu, Jinhui Feng, Peter C. K. Lau* and Dunming Zhu*. Substrate profiling of cyclohexylamine oxidase and its mutants reveals new biocatalytic potential in deracemization of racemic amines. Appl. Microbiol. Biotechnol [J]. 2014, 98(4),1681-1689. (IF5year =3.848)
10、Guangyue Li, Jie Ren, Qiaqing Wu*, Jinhui Feng, Dunming Zhu*, Yanhe Ma. Identification of a marine NADPH-dependent aldehyde reductase for chemoselective reduction of aldehydes. Journal of molecular catalysis B, Enzymatic [J], 2013, 90,17-22. (IF5year =2.786)
11、Li GuangYue,Yang XiuFen,Zeng HongMei,Mao JianJun,Liu Hua,Zhang YunHua,Qiu, DeWen* Stable isotope labelled mass spectrometry for quantification of the relative abundances for expressed proteins induced by PeaT1. Science China Life Sciences [J], 2010, 53(12),1410-7. (IF5year =2)
12、Zhoutong Sun?, Guangyue Li?, Adriana Ilie?, Manfred T. Reetz *. Exploring the substrate scope of mutants derived from the robust alcohol dehydrogenase TbSADH. Tetrahedron Letters[J]. 2016. 57, 3648-3651. (IF5year =2.026).
13、Peiyuan Yao, Peiqian Cong, Rui Gong, Jinlong Li, Guangyue Li, Jie Ren, Jinhui Feng, Jianping Lin, Peter C. K. Lau,* Qiaqing Wu,* and Dunming Zhu*.Biocatalytic Route to Chiral 2-Substituted-1, 2, 3, 4-tetrahydroquinolines Using Cyclohexylamine Oxidase Muteins. ACS Catalysis[J]. 2018, 8, 1648-1652. (IF5year =10.945)..
14、Ge Qu, Richard Lonsdale, Peiyuan Yao, Guangyue Li, Beibei Liu, Manfred T. Reetz,* and Zhoutong Sun*. Methodology Development in Directed Evolution: Exploring Options when Applying Triple‐Code Saturation Mutagenesis. ChemBioChem[J]. 2018, 19, 239-246. (IF5year =2.751).
15、Jian-bo Wang, Guangyue Li, Manfred T. Reetz*. Enzymatic site-selectivity enabled by structure-guided directed evolution. Chemical Communications [J]. 2017, 53, 3916-3928. (IF5year =6.238)
16、Zhoutong Sun, Richard Lonsdale, Adriana Ilie, Guangyue Li, Jiahai Zhou, and Manfred T. Reetz. Catalytic Asymmetric Reduction of Difficult-to-Reduce Ketones:Triple-Code Saturation Mutagenesis of an Alcohol Dehydrogenase. ACS Catalysis [J]. 2016, 6(3), 1598?1605. (IF5year =10.945)
17、Zhoutong Sun, Richard Lonsdale, Lian Wu, Guangyue Li, Aitao Li, Jianbo Wang, Jiahai Zhou, and Manfred T. Reetz. Structure-Guided Triple-Code Saturation Mutagenesis: Efficient Tuning of the Stereoselectivity of an Epoxide Hydrolase. ACS Catalysis [J]. 2016, 6, 1590?1597. (IF5year =10.945)
18、Zhoutong Sun, Richard Lonsdale, Guangyue Li and Manfred T. Reetz. Comparing Different Strategies in Directed Evolution of Enzyme Stereoselectivity: Single versus Double Code Saturation Mutagenesis. Chembiochem [J]. 2016, 17, 1865-1872. (IF5year =2.751)