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生物杀菌剂的创制与应用创新团队

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1zplay比分网研究员, 博士生导师, 1zplay电竞比分网“生物杀菌创制与应用”创新团队首席科学家,1zplay电竞比分网青年英才“A”类入选者。2003-2007年就读于河北农业大学生命学院并获得学士学位,2007-2010年就读于1zplay比分网并获得硕士学位,2010-2014年就读于中国科学院天津工业生物技术研究所并获得博士学位。2014-2017年在德国马普煤炭所Manfred.T.Reetz教授实验室从事博士后研究。2017年11月回国,入职1zplay比分网。主要从事线虫共生细菌中抗菌活性物质的发掘、代谢途径优化以及代谢途径中关键酶的定向改造等研究。在JACS、ACS Catalysis、Chemical Science、Chemical Communications等学术期刊上发表文章20多篇。

已发表文章

1.Li, G#.; Qin, Y#.; Fontaine, N. T#.; Ng Fuk Chong, M.; Maria‐Solano, M. A.; Feixas, F.; Cadet, X. F.; Pandjaitan, R.; Garcia‐Borràs, M.; Cadet, F., Machine Learning Enables Selection of Epistatic Enzyme Mutants for Stability Against Unfolding and Detrimental Aggregation.  ChemBioChem .  2020, 21,1-12. (IF5year ="2.5)

2. 李广悦, 杨秀芬. 嗜线虫致病杆菌抗菌代谢产物 Xcn1 的研究进展. 中国生物防治学报 2020, 36 (1), 1-8.

3. 段佳琪,李琲琲,任杰,邱德文,李广悦*.一种脱氧雪腐镰刀菌烯醇制备工艺的研究.生物技术通报. 2020, 36(10):1-8.

4. Dong, Y.; Li, X.; Duan, J.; Qin, Y.; Yang, X.; Ren, J.; Li, G*. Improving the Yield of Xenocoumacin 1 Enabled by In Situ Product Removal. ACS omega.  2020, 5(32), 20391–20398. (IF5year ="2.6)

5. Jiaqi Duan, Beibei Li, Youcai Qin, Yijie Dong, Jie Ren and Guangyue Li*. Recent progress in directed evolution of stereoselective monoamine oxidases. Bioresources and Bioprocessing [J]. 2019, 6: 1-11.

6.Guangyue Li, Yijie Dong, and Manfred T. Reetz*. Can Machine Learning Revolutionize Directed Evolution of Selective Enzymes? Adv. Synth. Catal [J]. 2019, 361, 2377 – 2386. (IF5year ="5.029)

7.Yijie Dong#, Peiyuan Yao#, Yunfeng Cui, Qiaqing Wu, Dunming Zhu*, Guangyue Li*and Manfred T. Reetz*. Manipulating the stereoselectivity of a thermostable alcohol dehydrogenase by directed evolution for efficient asymmetric synthesis of arylpropanols. Biological Chemistry [J]. 2019,400(3), 313-321. (IF5year ="3.2)

8.Guangyue Li, Marc Garcia-Borràs, Maximilian J. L. J. Fürst, Adriana Ilie, Marco W. Fraaije, K. N. Houk*and Manfred T. Reetz*. Overriding Traditional Electronic Effects in Biocatalytic Baeyer-Villiger Reactions by Directed Evolution. JACS [J]. 2018, 140, 10464?10472. (IF5year ="14.35)

9.Peiyuan Yao, Peiqian Cong, Rui Gong, Jinlong Li, Guangyue Li, Jie Ren, Jinhui Feng, Jianping Lin, Peter C. K. Lau,* Qiaqing Wu,* and Dunming Zhu*.Biocatalytic Route to Chiral 2-Substituted-1, 2, 3, 4-tetrahydroquinolines Using Cyclohexylamine Oxidase Muteins. ACS Catalysis[J]. 2018, 8, 1648-1652. (IF5year ="10.945).

10.Frédéric Cadet, Nicolas Fontaine, Guangyue Li, Joaquin Sanchis, Matthieu Ng Fuk Chong,Rudy Pandjaitan, Iyanar Vetrivel , Bernard Offmann & Manfred T. Reetz. A machine learning approach for reliable prediction of amino acid interactions and its application in the directed evolution of enantioselective enzymes. Scientific reports [J]. 2018, 8:16757. (IF5year ="4.12)

11.Ge Qu, Richard Lonsdale, Peiyuan Yao, Guangyue Li, Beibei Liu, Manfred T. Reetz,* and Zhoutong Sun*. Methodology Development in Directed Evolution: Exploring Options when Applying Triple‐Code Saturation Mutagenesis. ChemBioChem[J]. 2018, 19, 239-246. (IF5year ="2.751).

12.Guangyue Li#, Peiyuan Yao#, Rui Gong#, Jinlong Li, Pi Liu,Richard Lonsdale, Qiaqing Wu, Jianping Lin*,Dunming Zhu* and Manfred T. Reetz*. Simultaneous engineering of an enzyme’s entrance tunnel and active site: The case of monoamine oxidase MAO-N. Chemical Science [J]. 2017, 8,4093-4099. (IF5year="8.733)

13.Guangyue Li, Miguel A. Maria-Solano, Adrian Romero-Rivera, Sílvia Osuna* and Manfred T. Reetz*. Inducing High Activity of a Thermophilic Enzyme at Ambient Temperatures by Directed Evolution. Chemical Communications [J]. 2017, 53,9454-9457. (IF5year ="6.238)

14.Guangyue Li, M.J. L. J. Fürst, H. R. Mansouri, A. K. Ressmann, A. Ilie, F. Rudroff, M. D. Mihovilovic*, M.W. Fraaije* and M. T. Reetz*. Manipulating the stereoselectivity of the thermostable Baeyer–Villiger monooxygenase TmCHMO by directed evolution. Organic & Biomolecular Chemistry [J]. 2017,15,9824-9829. (IF5year ="3.56)

15.Guangyue Li, Jianbo Wang and Manfred T. Reetz*. Biocatalysts for the pharmaceutical industry generated by structure-guided directed evolution ofstereoselective enzymes. Bioorganic & Medicinal Chemistry [J]. 2017, 7(26), 1241-1251 (IF5year="2.88)

16.Jian-bo Wang, Guangyue Li, Manfred T. Reetz*. Enzymatic site-selectivity enabled by structure-guided directed evolution. Chemical Communications [J]. 2017, 53, 3916-3928. (IF5year ="6.238)

17.Guangyue Li#, Hui Zhang#, Zhoutong Sun, Xinqi Liu and Manfred T. Reetz*. Multiparameter Optimization in Directed Evolution: Engineering Thermostability, Enantioselectivity, and Activity of an Epoxide Hydrolase. ACS Catalysis [J]. 2016, 6(6), 3679?3687. (IF5year ="10.945)

18.Guangyue Li and Manfred T. Reetz*. Learning Lessons from Directed Evolution of Stereoselective Enzymes. Org. Chem. Front [J]. 2016. 3, 1350-1358 (IF5year ="4.96)

19.Guangyue Li#, Peiyuan Yao#, Peiqian Cong, Jie Ren, Lei Wang, Jinhui Feng, Peter C.K. Lau, Qiaqing Wu*,and Dunming Zhu*. New recombinant cyclohexylamine oxidase variants for deracemization of secondary amines by orthogonally assaying designed mutants with structurally diverse substrates. Scientific reports [J]. 2016,6:24973. (IF5year ="4.847) 

20.Zhoutong Sun?Guangyue Li?, Adriana Ilie?, Manfred T. Reetz *. Exploring the substrate scope of mutants derived from the robust alcohol dehydrogenase TbSADH. Tetrahedron Letters[J]. 2016. 57, 3648-3651. (IF5year ="2.02).

21.Zhoutong Sun, Richard Lonsdale, Adriana Ilie, Guangyue Li, Jiahai Zhou, and Manfred T. Reetz. Catalytic Asymmetric Reduction of Difficult-to-Reduce Ketones:Triple-Code Saturation Mutagenesis of an Alcohol Dehydrogenase. ACS Catalysis [J]. 2016, 6(3), 1598?1605. (IF5year ="10.945)

22.Zhoutong Sun, Richard Lonsdale, Lian Wu, Guangyue Li, Aitao Li, Jianbo Wang, Jiahai Zhou, and Manfred T. Reetz. Structure-Guided Triple-Code Saturation Mutagenesis: Efficient Tuning of the Stereoselectivity of an Epoxide Hydrolase. ACS Catalysis [J]. 2016, 6, 1590?1597. (IF5year ="10.945)

23.Zhoutong Sun, Richard Lonsdale, Guangyue Li and Manfred T. Reetz. Comparing Different Strategies in Directed Evolution of Enzyme Stereoselectivity: Single versus Double Code Saturation Mutagenesis. Chembiochem [J]. 2016, 17, 1865-1872. (IF5year ="2.751)

24.Guangyue Li#, Jie Ren#, Peiyuan Yao,Yitao Duan, Hailing Zhang, Qiaqing Wu, Jinhui Feng, Peter CK Lau*, Dunming Zhu*. Deracemization of 2-Methyl-1, 2, 3, 4-tetrahydroquinoline Using Mutant Cyclohexylamine Oxidase Obtained by Iterative Saturation Mutagenesis. ACS Catalysis [J]. 2014, 4,903-908. (IF5year ="9.174) 

25.Guangyue Li, Jie Ren, Hiroaki Iwaki, Dalong Zhang, Yoshie Hasegawa, Qiaqing Wu, Jinhui Feng, Peter C. K. Lau* and Dunming Zhu*. Substrate profiling of cyclohexylamine oxidase and its mutants reveals new biocatalytic potential in deracemization of racemic amines. Applied Microbiology and Biotechnology [J]. 2014, 98(4),1681-1689. (IF5year ="3.848)

26.Guangyue Li, Jie Ren, Qiaqing Wu*, Jinhui Feng, Dunming Zhu*, Yanhe Ma. Identification of a marine NADPH-dependent aldehyde reductase for chemoselective reduction of aldehydes. Journal of molecular catalysis B, Enzymatic [J], 2013, 90,17-22. (IF5year ="2.786)

27.Li GuangYue,Yang XiuFen,Zeng HongMei,Mao JianJun,Liu Hua,Zhang YunHua,Qiu, DeWen* Stable isotope labelled mass spectrometry for quantification of the relative abundances for expressed proteins induced by PeaT1. Science China Life Sciences [J], 2010, 53(12),1410-7. (近五年平均IF=2.88)

28.梁颖博, 李泽, 邱德文, 曾洪梅, 李广悦, 杨秀芬. 本生烟响应蛋白激发子 PevD1 的差异表达基因鉴定与分析. 中国农业科学, 2019,52(21), 3794-3805.

29.刘 权, 李广悦, 曾洪梅, 杨秀芬, 邱德文. 微生物蛋白激发子PeaT1的获得及诱导水稻抗旱性的初步研究. 中国农业科技导报, 2009, 11( 3), 51- 55.

30.刘权,李广悦,曾洪梅,杨秀芬,邱德文.蛋白激发子PeaT1的高密度发酵及生理功能检测.生物技术通报,2009,8,162-165.